superoxide dismutase sod  (Elabscience Biotechnology)

Journal: International Dental Journal

Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways

doi: 10.1016/j.identj.2026.109472

Figure Lengend Snippet: Oxidative stress markers in the study population, stratified by obesity presence, before and after non-surgical periodontal treatment. Total ROS (A and G), cytosolic superoxide (B and H) and mitochondrial superoxide (C and I) in leukocytes, and protein levels of SOD1 (D and J) in PBMCs normalized to the loading control actin with corresponding WB images (E), and serum total antioxidant capacity (F and K). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. ** P < .01 when comparing baseline vs 12 wk. # P < .05 when comparing groups with vs without obesity. DCFH, diclorodihidrofluoresceína; dHE, dihydroethidium; MitoSOX, Mitochondrial Superoxide Indicator; ns, not significant; PBMCs, peripheral blood mononuclear cells; ROS, reactive oxygen species; SOD1, superoxide dismutase 1.

Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID:AB_2544716), rabbit polyclonal anti-IRE1α (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1-250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID:AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2α) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID:AB_476738).

Techniques: Control, Whisker Assay

Journal: Journal of Pharmaceutical Analysis

Article Title: Naringenin boosts Parkin-mediated mitophagy via estrogen receptor alpha to maintain mitochondrial quality control and heal diabetic foot ulcer

doi: 10.1016/j.jpha.2025.101333

Figure Lengend Snippet: Naringenin (NAR) alleviates oxidative stress and inhibition of autophagy induced by high glucose (HG). (A) HaCaT cells were treated with NAR (0.12, 0.37, 1.1, and 3.3 μM) for 24 h after being induced with HG (50 mM) for 24 h. Then, HaCaT cells were incubated with dihydroethidium (DHE) probes for 30 min, and the reactive oxygen species (ROS) levels in each group were detected using inverted fluorescence microscope. (B) HaCaT cells were incubated with DHE probes for 30 min, and the fluorescence intensity of DHE at different excitation wavelengths was subsequently detected using a multifunctional microplate detection platform. The 535 nm/370 nm ratio was quantified to assess ROS levels. (C) HaCaT cells were incubated with 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) dye for 30 min, and the JC-1 fluorescence was then examined using inverted fluorescence microscope. JC-1 aggregates (red) represent mitochondria with normal membrane potential, while JC-1 monomers (green) represent mitochondria with membrane potential depolarization. (D) The levels of oxidative stress-related proteins, including nicotinamide adenine dinucleotide phosphate (NAD(P)H):quinone oxidoreductase 1 (NQO1), superoxide dismutase 2 (SOD2), SOD1, and glutaredoxin 1 (GRX1), in HaCaT cells were measured by Western blotting. (E) Representative immunohistochemistry (IHC) staining images of NQO1 in wounds of mice at day 9 post-puncture. (F) The levels of sequestosome-1 (P62) and microtubule-associated proteins 1A/1B light chain 3C (LC3) in HaCaT cells were detected by Western blotting. (G) HaCaT cells were immunostained with antibody against LC3 and visualized using confocal microscope. (H) The LC3 puncta in the cells were quantified by ImageJ. Data are presented as mean ± standard deviation (SD). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, compared with the control group. GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: After blocking with 5% fat-free milk in Tris-buffered saline containing 0.1% Tween 20, the PVDF membrane was incubated with primary antibodies against β-actin (Santa Cruz Biotechnology), β-tubulin (Biodragon, Suzhou, China), p-H2A histone family member X (p-γH2AX) (Bioss, Beijing, China), adenosine triphosphate (ATP) synthase F1 subunit alpha (ATP5F1A) (Sangon Biotech Co., Ltd.), Cav-1 (Sangon Biotech Co., Ltd.), cytochrome C oxidase subunit 4 (COX IV) (Proteintech), dynamin-1-like protein (DRP1) (Sangon Biotech Co., Ltd.), ERα (Proteintech), ERβ (Sangon Biotech Co., Ltd.), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Proteintech), glutaredoxin 1 (GRX1) (Proteintech), histone H3 (Proteintech), heat shock protein 60 (HSP60) (Sangon Biotech Co., Ltd.), LaminB1 (Sangon Biotech Co., Ltd.), LC3 (MBL), mitofusin 2 (MFN2) (Proteintech), cytochrome C oxidase subunit 2 (MT-CO2) (Sangon Biotech Co., Ltd.), nicotinamide adenine dinucleotide (NADH) dehydrogenase (ubiquinone) iron-sulfur protein 4 (Ndufs4) (Sangon Biotech Co., Ltd.), nuclear factor-κB (NF-κB) (Proteintech), NQO1 (Sangon Biotech Co., Ltd.), nuclear respiratory factor 1 (NRF1) (Proteintech), cyclin-dependent kinase inhibitor 1A (P21) (Proteintech), sequestosome-1 (P62) (Proteintech), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) (Proteintech), PTEN-induced putative kinase 1 (PINK1) (Novus Biologicals, Littleton, CO, USA), p-NF-κB (S536) (Cell Signaling Technology), p-PINK1 (S228) (Thermo Fisher Scientific Inc.), succinate dehydrogenase complex flavoprotein subunit A (SDHA) (Proteintech), superoxide dismutase 1 (SOD1) (Proteintech), SOD2 (Proteintech), transcription factor A mitochondrial (TFAM) (Proteintech), translocase of inner mitochondrial membrane 23 (Tim23) (BD Biosciences, San Jose, CA, USA), Tomm20 (Proteintech), and voltage-dependent anion-selective channel protein 1 (VDAC1) (Sangon Biotech Co., Ltd.) overnight at 4 °C.

Techniques: Inhibition, Incubation, Fluorescence, Microscopy, Membrane, Western Blot, Immunohistochemistry, Standard Deviation, Control